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制限酵素類 |
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5324室(-20冷凍庫最上段) |
5325室 |
5323室 |
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AflII ApaI ApaLI |
AatII Aor51HI ApaI AvaI AvaII |
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BamHI BglII |
BalI BamHI BglI BglII BstPI BstXI |
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Csp45I |
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EcoRI EcoRV |
EcoRI EcoRV
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HindIII |
HhaI HincII HindIII |
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KpnI |
KpnI |
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MluI MboI |
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NdeI NheI NotI |
NarI NcoI NdeI NheI NruI |
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PstI |
PstI PvuII |
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SalI ScaI SmaI SnaBI SpeI SphI |
SacI SacII Sau3AI ScaI SfiI SmaI SphI SplI StuI |
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TthHB8I |
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XbaI XhoI |
XbaI XhoI |
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Vectors
and E. coli
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5324室(-20degC冷凍庫最上段と下から二段目) |
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pMKITNeo pCMV-Tag3a,b,c pQBI25 |
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pNGFPa,b,c pCGFPa |
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pBluescriptII KS- pUC119 pGEX4T1 pET16b |
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JM109 BL21(DE3) |
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PCR,Sequence,etc |
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5324室(-20degC冷凍庫最下段) |
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ThermoSequenaseII
(Pre-mixA and B) |
DYEnamic ET Terminator Cycle
Sequencing Kit (Sequence用；Amersham
Pharmacia) |
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Platinum
Pfx DNA polymerase dNTP
mix (2.5mM x 4) |
PLATINUM
Taq PCRx Takara
Ex Taq Expand
High-Fidelity PCR System DNA
Polymerase, Klenow Fragment |
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Takara
DNA ligation kit Ver2 Takara
DNA blunting kit |
Takara
Ligation Kit Rapid DNA Ligation Kit
(Roche) DNA Blunting Kit ( Takara ) Mutan-Super Express Km Kit (Site-Directed
Mutagenesis用；Takara) |
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Mung
Bean Nuclease Alkaline
Phosphatase (Calf intestine) |
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DIG DNA Labelling and Detection Kit (Non-RI DNAラベリング用；Roche) |
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T7
primer T3
primer M13
(-40) primer 種々の合成プライマー 種々の合成ペプチド |
Bca BEST Primer M13-47 (Takara)
Bca BEST Primer RV-M (Takara) |
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SDS-PAGE LMW marker Precision prestained marker |
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HRP-標識抗ウサギIgG HRP-標識抗マウスIgG 種々の抗体 |
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MBS Biotin-maleimide NHS-biotin Dithionitrobenzoic acid complete adjuvant |
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ここからは、冷蔵庫と室温品 |
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5324室の冷蔵庫 |
5324室温品 |
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1kb DNA ladder (x1,x10) glass milk＋Wash液 |
Quantum prep plasmid purification kit |
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protein A-Sepahrose fast flow Glutathione Sepharose Qiagen Ni-agarose |
advantec 0.2micron filter Millipore PVDF microfilter |
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NHS-Sepharose-FF (cartridge-type) NHS-Sepharose-FF (bottle-type) activated thiol-sepharose Superdex30 prep |
Sephadex G-25 Sephadex G-50 Sephadex G-75 |
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BacVector3000 baculo triplecut virus
kit |
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FITC-anti-rabbit IgG FITC-anti-mouse IgG TRITC-anti-rabbit IgG TRITC-anti-mouse IgG |
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Bovine serum albumin |
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SuperSignalWestPico |
PVDF膜 Nitrocellulose膜 |
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