Professor
Department of Pathophysiology
Meiji Pharmaceutical University
2-522-1 Noshio, Kiyose, Tokyo 204-8588 Japan
Phone & fax: 81-42-495-8502
mail: kishiba@my-pharm.ac.jp
Education:
1981, 3: Graduated from School of Medicine, Tokyo Medical and Dental University
Occupation
1981, 5~1983, 5: Residency program at Department of Internal Medicine, School of Medicine, Tokyo Medical and Dental University
1983, 6~1984, 5: Staff Physician at Renal Unit of Ohkubo Hospital
1984, 6~1985, 5: Staff Physician at Tsuchiura Mutual Hospital
1985, 6~1986, 7: Fellow at Internal Medicine at Tokyo Medical and Dental University
1986, 8~1987, 6: Staff Physician at Hokushin General Hospital
1987, 7~1990, 4: Research Fellow at Renal Unit and Endocrine Department of University of California, San Francisco.
1990, 5~1993, 7: Attending physician at Department of Internal Medicine at Tokyo Medical and Dental University
1993, 8~1998, 5: Deputy Chief at Renal Unit of Ohme General Hospital.
1998, 6~2003, 3: Associate professor at Department of Pharmacology, Jichi Medical School
2003, 4~2004, 3: Associate professor at Department of Nephrology, Jichi Medical School
2004, 4~2007, 3: Head, Division of Molecular Biology Research, Clinical Research Center, Chiba-East National Hospital, National Hospital Organization.
2007, 4~ present: Professor, Department of Pathophysiology, Meiji Pharmceutical University
Academic activity:
2000~present: Fellow of Japanese Society of Nephrology
Members of Pharmaceutical Society of Japan, Japanese Society of AppliedTherapeutics, American Society of Nephrology
Editorial Board of Am. J. Physiol.-regulatory, integrative and comparative physiology
Publications:
(I) Original Articles
1. Ishibashi K, Sasaki S, Shiigai T, Takeuchi J: Intracellular electrical potentials in the rabbit collecting ducts perfused in vitro. Jpn J Physiol, 36(4):671-81. 1986
2. Ishibashi K, Sasaki S, Yoshiyama N, Shiigai T, Takeuchi J: Generation of pH gradient across the rabbit collecting duct segments perfused in vitro. Kidney Int. 31(4):930-6. 1987
3. Sasaki S, Ishibashi K, Yoshiyama N, Shiigai T: KCl co-transport across the basolateral membrane of rabbit renal proximal straight tubules. J Clin Invest. 81(1):194-9. 1988
4. Ishibashi K, Sasaki S, Yoshiyama N: Intracellular chloride activity of rabbit proximal straight tubule perfused in vitro. Am J Physiol, 255:F49-56. 1988
5. Kuwahara M, Ishibashi K, Krapf R, Rector FC Jr, Berry CA. Effect of lumen pH on cell pH and cell potential in rabbit proximal tubules. Am J Physiol, 256:F1075-83. 1989
6. Ishibashi K, Rector FC Jr, Berry CA: Chloride transport across the basolateral membrane of rabbit proximal convoluted tubules. Am J Physiol. 258:F1569-78. 1990
7. Sasaki S, Ishibashi K, Marumo F: Transcellular chloride transport in the proximal tubules: mechanisms and physiological significance. Kidney Int Suppl. 33:S109-12. 1991
8. Ishibashi K, S. Sasaki, H. Sakamoto, Y. Nakamura, T. Hata, T. Nakamura, and F. Marumo: Hepatocyte growth factor is a paracrine factor for renal epithelial cells: stimulation of DNA synthesis and Na,K-ATPase activity. Biochem. Biophys. Res. Commun. 182: 960-965, 1992
9. Ishibashi K, S. Sasaki, H. Sakamoto, Y. Hoshino, T. Nakamura, and F. Marumo: Expressions of receptor gene for hepatocyte growth factorin kidney after unilateral nephrectomy. Biochem. Biophys. Res. Commun. 187: 1454-1459, 1992
10. Matsuda O, Makiguchi K, Ishibashi K, Chida Y, Ida T, Matsuda K, Tomita K, Marumo F, Hiruma M: Long-term effects of steroid treatment on nephritic syndrome associated with Kimura’s disease and a review of the literature. Clin Nephrol 37: 119-123, 1992
11. Sasaki S, Ishibashi K, Nagai T, Marumo F: Regulation mechanisms of intracellular pH of Xenopus laevis oocyte. Biochim Biophys Acta. 1137:45-51, 1992
12. Ishibashi K, Rector FC Jr, Berry CA: Role of Na-dependent Cl/HCO3 exchange in basolateral Cl transport of rabbit proximal tubules. Am J Physiol. 264:F251-8, 1993
13. Ishibashi K: Intracellular Cl- activity in rabbit proximal convoluted tubule perfused in vitro: regulation by sodium and effects of anion transport inhibitors. Jpn J Physiol. 43:585-97, 1993.
14. Ishibashi K, S. Sasaki, T. Akiba, and F: Marumo. Expression of bone morphogenic protein 7 mRNA in MDCK cells. Biochem. Biophys. Res. Commun. 193: 235-239, 1993.
15. Ishibashi K, Sasaki S, Uchida S, Imai T, Marumo F: Tissue expression of mRNA of chloride channel from MDCK cells and its regulation by protein kinases. Biochem. Biophys. Res. Commun.192: 561-7, 1993.
16. Ishibashi K, S. Sasaki, K. Fushimi, S. Uchida, M. Kuwahara, H. Saito, T. Furukawa, K. Nakajima, Y. Yamaguchi, T. Gojobori, and F. Marumo: Molecular cloning and expression of a member of the aquaporin family with permeability to glycerol and urea in addition to water expressed at the basolateral membrane of kidney collecting duct cells. Proc. Natl. Acad. Sci. USA 91: 6269-6273, 1994.
17. Ishibashi K, S. Sasaki, F. Saito, T. Ikeuchi, and F. Marumo: Structure and chromosomal localization of a human water channel (AQP3) gene. Genomics 27, 352-354, 1995.
18. Inase N, Fushimi K Ishibashi K, Uchida S, Ichioka M, Sasaki S, Marumo F: Isolation of human aquaporin 3 gene. J Biol Chem. 270: 17913-6, 1995.
19. Yamamoto-T, Sasaki-S, Fushimi-K, Ishibashi-K, Yaoita-E, Kawasaki-K, Marumo-F, Kihara-I: Vasopressin increases AQP-CD water channel in apical membrane of collecting duct cells in Brattleboro rats. Am-J-Physiol.268: C1546-51, 1995.
20. Ishibashi K, S. Sasaki, K. Fushimi, T. Yamamoto, M. Kuwahara, and F. Marumo: Immunolocalization and effect of dehydration on AQP3, a basolateral water channel of kidney collecting ducts. Am. J. Physiol. 272: F235-F241, 1997.
21. Ishibashi K, M. Kuwahara, Y. Gu, Y. Kageyama, A. Tohsaka, F. Suzuki, F. Marumo, and S. Sasaki. : Cloning and functional expression of a new water channel abundantly expressed in the testis permeable to water, glycerol, and urea. J. Biol. Chem. 272:20782-20786, 1997.
22. Ishibashi K, M. Kuwahara, Y. Kageyama, A. Tohsaka, F. Marumo, and S. Sasaki.: Cloning and functional expression of a second new water channel abundantly expressed in testis. Biochem. Biophys. Res. Commun. 237: 714-718, 1997.
23. Tanaka-M, Inase-N, Fushimi-K, Ishibashi-K, Ichioka-M, Sasaki-S, Marumo-F : Induction of aquaporin 3 by corticosteroid in a human airway epithelial cell line. Am. J. Physiol. 273: L1090-5, 1997.
24. Kuwahara-M, Gu-Y, Ishibashi-K, Marumo-F, Sasaki-S : Mercury-sensitive residues and pore site in AQP3 water channel. Biochemistry. 36(46): 13973-8, 1997.
25. Yamamoto-T, Sasaki-S, Fushimi-K, Ishibashi-K, Yaoita-E, Kawasaki-K, Fujinaka-H, Marumo-F, Kihara-I : Expression of AQP family in rat kidneys during development and maturation. Am-J-Physiol. 272: F198-204, 1997.
26. Hayashi-M, Yamaji-Y, Monkawa-T, Yoshida-T, Tsuganezawa-H, Sasamura-H, Kitajima-W, Sasaki-S, Ishibashi-K, Maurmo-F, Saruta-T: Expression and localization of the water channels in human autosomal dominant polycystic kidney disease. Nephron. 75(3): 321-6, 1997.
27. Ishibashi K, M. Kuwahara, Y. Gu, Y. Tanaka, F. Marumo, and S. Sasaki. : Cloning and functional expression of a new aquaporin(AQP9) abundantly expressed in the peripheral leukocytes permeable to water and urea, but not to glycerol. Biochem. Biophys. Res. Commun. 244: 268-274, 1998.
28. Ishibashi K and Marumo F: Molecular cloning of a DEG/ENaC Sodium channel cDNA from human testis. Biochem. Biophys. Res. Commun. 245: 589-593, 1998.
29. Ishibashi K, S. Sasaki, and F. Marumo. : Molecular cloning of a new sodium bicarbonate cotransporter cDNA from human retina. Biochem. Biophys. Res. Commun. 246: 535-538, 1998.
30. Ishibashi K, Yamauchi K, Kageyama Y, Saito-Ohara F, Ikeuchi T, Marumo F, and Sasaki S: Molecular characterization of human Aquaporin-7 gene and the chromosomal mapping. Biochim. Biophys. Acta,1399:62-66, 1998.
31. Michio Kuwahara, Ishibashi K, Y. Gu, S. Sasaki, and F. Marumo : Functional expression of the first water channel from C. elegans. Am. J. Physiol. 275: C1459-1469, 1998.
32. N. Koyama, Ishibashi K, M. Kuwahara, N. Inase, M. Ichioka, S. Sasaki, F. Marumo. Cloning and functional expression of human aquaporin8 cDNA and analysis of its gene. Genomics 54: 169ｰ172､ 1998.
33. Ishibashi K, K. Miyamoto, Y. Taketani, K. Morita, E. Takeda, S. Sasaki, and M. Imai : Molecular cloning of a second human stanniocalcin homologue (STC2). Biochem. Biophys. Res. Commun.250: 252-258, 1998
34. Ko SB, Uchida S, Naruse S, Kuwahara M, Ishibashi K, Marumo F, Hayakawa T, Sasaki S. Cloning and functional expression of rAOP9L a new member of aquaporin family from rat liver. Biochem Mol Biol Int 47:309-18, 1999.
35. F. Suzuki, Ishibashi K, S. Yuasa: Immunohistochemical localization of a water channel, aquaporin (AQP7), in the rat testis. Cell Tissue Res. 295:279-85, 1999.
36. Ishibashi K and Masashi Imai: Identification of four new members of the rat prolactin/growth hormone gene family. Biochem. Biophys. Res. Commun. 262: 575-578, 1999.
37. Ishibashi K, Suzuki M, Imai M: Molecular cloning of a novel form (two-repeat) protein related to voltage-gated sodium and calcium channels. Biochem Biophys Res Commun. 270:370-6, 2000.
38. Suzuki M, Ishibashi K, Ooki G, Tsuruoka S, Imai M: Electrophysiologic Characteristics of the Ca-Permeable Channels, ECaC and CaT, in the Kidney. Biochem Biophys Res Commun. 274:344-349, 2000.
39. Ohki G, Miyoshi T, Murata M, Ishibashi K, Imai M, and Suzuki M: A Calcium-Activated Cation Current by an Alternatively Spliced Form of TRP3 in the Heart. J Biol Chem. 275(50):39055-60, 2000.
40. Ishibashi K, Imai M, Sasaki S: Cellular localization of aquaporin 7 in the rat kidney. Exp. Nephrol. 8(4-5): 252-257, 2000.
41. Kageyama Y, Ishibashi K, Hayashi T, Xia G, Sasaki S, Kihara K: Expression of aquaporin 7 and 8 in developing rat testis. Andrologia 33: 165-169, 2001.
42. Ishibashi K, Makoto Suzuki, Sei Sasaki, and Masashi Imai: Identification of a new multigene four-transmembrane family (MS4A) related to CD20, HTm4 and beta subunit of the high-affinity IgE receptor. GENE 264: 87-93, 2001.
43. Hoenderop JG, Muller D, Van Der Kemp AW, Hartog A, Suzuki M, Ishibashi K, Imai M, Sweep F, Willems PH, Van Os CH, Bindels RJ: Calcitriol controls the epithelial calcium channel in kidney. J Am Soc Nephrol. 12:1342-9, 2001.43-2. Calcitriol Controls the Epithelial Calcium Channel in Kidney
JOOST G. J. HOENDEROP*, DOMINIK MULLER*, ANNEMIETE W. C. M. VAN DER KEMP*, ANITA HARTOG*, MAKOTO SUZUKI§, KENICHI ISHIBASHI§, MASASHI IMAI§, FRED SWEEP‡, PETER H. G. M. WILLEMS†, CAREL H. VAN OS* and RENE J. M. BINDELS*J Am Soc Nephrol 12:1342-1349, 2001
44. Suzuki M, Ohki G, Ishibashi K, Imai M: A single amino acid mutation results in a rapid inactivation of epithelial calcium channels. Biochem Biophys Res Commun. 291:278-85, 2002.
45. Morinaga T, Nakakoshi M, Hirao A, Imai M, Ishibashi K: Mouse aquaporin 10 gene (AQP10) is a pseudogene. Biochem Biophys Res Commun. 294:630-4, 2002.
46. Suzuki M, Ohki G, Mochizuki T, Somlo S, Ishibashi K, Imai M: Opening of ligand-gated cation channel families by calpain inhibitors. FEBS Lett. 517:219-24, 2002.
47. Ishibashi K, Morinaga T, Kuwahara M, Sasaki S, Imai M: Cloning and identification of a new member of water channel (AQP10) as an aquaglyceroporin. Biochim Biophys Acta. 1576:335-40, 2002.
48. Shigeru B. H. Ko, Xiang Luo, Henrik Hager , Alexandra Rojek, Joo Young Choi, Christoph Licht Makoto Suzuki, Shmuel Muallem, Soren Nielsen and Kenichi Ishibashi: AE4 is a DIDS-Sensitive Cl-/HCO3- exchanger in the basolateral membrane of the rat and mouse renal cortical collecting duct and the submandibular gland duct. Am. J. Physiol. 283: C1206-1218, 2002.
49. Shigeru B. H. Ko, Nikolay Shcheynikov, Joo Young Choi, Xiang Luo, Kenichi Ishibashi, Philip J Thomas, Joo Young Kim, Kyung Hwan Kim, Min Goo Lee, Satoru Naruse and Shmuel Muallem: A molecular mechanism for aberrant CFTR-dependent HCO3- transport in cystic fibrosis. EMBO J 21:5662-5672, 2002.
50. Shuichi Tsuruoka, Kenichi Ishibashi, Hisashi Yamamoto, Michi Wakaumi, Makoto Suzuki, George J Schwartz, Masashi Imai, Akio Fujimura: Functional analysis of ABCA8, a new drug transporter. Biochem Biophys Res Commun. 298: 41-45, 2002.
51. Ishibashi K, T Matsuzaki, K Takata and M Imai: Identification of a new member of type 1 Na/phosphate cotransporter in the rat kidney. Nephron-physiology. 94: 10-18, 2003 pdf
52. Takeda S, Takahashi M, Mizukami H, Kobayashi E, Takeuchi K, Hakamata Y, Kaneko T, Yamamoto H, Ito C, Ozawa K, Ishibashi K, Matsuzaki T, Takata K, Asano Y, and Kusano E: Successful Gene Transfer Using Adeno-Associated Virus Vectors into the Kidney: Comparison among Adeno-Associated Virus Serotype 1 to 5 Vectors In Vitro and In Vivo. Nephron Exp Nephrol. 96(4):e119-26, 2004.
53. Saito K, Kageyama Y,Okada Y, Kawakami S, Kihara K, Ishibashi K, Sasaki S: Localization of aquaporin-7 in human testis and ejaculated sperm: possible involvement in maintenance of sperm quality. J. Urol. 172: 2073-2076, 2004.
54. Li H, Kamiie J, Morishita Y, Yoshida Y, Yaoita E, Ishibashi K, Yamamoto T: Expression and Localization of Two Isoforms of AQP10 in Human Small Intestine. Biol Cell. 97:823-829, 2005.
55. Wakaumi M, Ishibashi K, Ando A, Kasanuki H, Tsuruoka S: Acute digoxin loading reduces ABCA8a mRNA expression in the mouse liver. Clinical and Experimental Pharmacology and Physiology, 32: 1034-1041, 2005.
56. Itoh T, Rai T, Kuwahara M, Ko SB, Uchida S, Sasaki S, Ishibashi K: Identification of a novel aquaporin, AQP12, expressed in pancreatic acinar cells. Biochem Biophys Res Commun. 330:832-838, 2005.
57. Morishita Y, Matsuzaki T, Hara-chikuma M, Andoo A, Shimono M, Matsuki A, Kobayashi K, Ikeda M, Yamamoto T, Verkman AS, Kusano E, Ookawara S, Takata K, Sasaki S , Ishibashi K: Disruption of aquaporin-11 produces polycystic kidneys following vacuolization of the proximal tubule. Mol. Cell. Biol. 25:7770-7779, 2005.
58. Nakakoshi M, Morishita Y, Usui K, Ohtsuki M, Ishibashi K: Identification of a keratinocarcinoma cell line expressing AQP3. Biol Cell. 98:95-100, 2006.
59. Yakata K, Hiroaki Y, Ishibashi K, Sohara E, Sasaki S, Mitsuoka K, Fujiyoshi Y: Aquaporin-11 containing a divergent NPA motif has normal water channel activity. Biochem Biophys Acta. 1768: 688-693, 2007.
60. Okada S, Misaka T, Tanaka Y, Matsumoto I, Ishibashi K, Sasaki S, Abe K. Aquaporin-11 knockout mice and polycystic kidney disease animals share a common mechanism of cyst formation. Faseb J 22: 3672-3684, 2008.
61. Hayakawa S, Mori M, Okuta A, Kamegawa A, Fujiyoshi Y, Yoshiyama Y, Mitsuoka K, Ishibashi K, Sasaki S, Hattori T, Kuwabara S. Neuromyelitis optica and anti-aquaporin-4 antibodies measured by an enzyme-linked immunosorbent assay. J Neuroimmunol, 196:181-187, 2008.
62. Tchekneva EE, Khuchua Z, Davis LS, Kadkina V, Dunn SR, Bachman S, Ishibashi K, Rinchik EM, Harris RC, Dikov MM, Breyer MD. Single amino acid substitution in aquaporin 11 causes renal failure. J Am Soc Nephrol. 19:1955-1964, 2008.
63. Ohta E, Itoh T, Nemoto T, Kumagai J, Ko SB, Ishibashi K, Ohno M, UchidaK, Ohta A, Sohara E, Uchida S, Sasaki S, Rai T. Pancreas-specific aquaporin12 null mice showed increased susceptibility to caerulein-induced acutepancreatitis.Am J Physiol Cell Physiol. 2009 Dec;297(6):C1368-7864. Ikeda M, Andoo A, Shimono M, Takamatsu N, Taki A, Muta K, Matsushita W, Uechi T, Matsuzaki T, Kenmochi N, Takata K, Sasaki S, Ito K, Ishibashi K. The NPC motif of aquaporin-11, unlike the NPA motif of known aquaporins, is essential for full expression of molecular function. J Biol Chem. 2011 Feb 4;286(5):3342-50.
65. Morishita Y, Hanawa S, Miki T, Sugase T, Sugaya Y, Chinda J, Iimura O, Tsunematsu S, Ishibashi K, Kusano E. The association of plasma prorenin level with an oxidative stress marker, 8-OHdG, in nondiabetic hemodialysis patients. Clin Exp Nephrol. 2011 Jan 15. 15(3):398-404
66. Morishita Y, Ohnishi A, Watanabe M, Ishibashi K, Kusano E. Establishment of acute kidney injury mouse model by 0.75% adenine ingestion. Ren Fail. 2011;33(10):1013-8.
67. Morishita Y, Watanabe M, Nakazawa E, Ishibashi K, Kusano E. The interaction of LFA-1 on mononuclear cells and ICAM-1 on tubular epithelial cells accelerates TGF-β1-induced renal epithelial-mesenchymal transition. PLoS One. 2011;6(8):e23267.
68.Ikeda M, Andoo A, Shimono M, Takamatsu N, Taki A, Muta K, Matsushita W, Uechi T, Matsuzaki T, Kenmochi N, Takata K, Sasaki S, Ito K, Ishibashi K. The NPC motif of aquaporin-11, unlike the NPA motif of known aquaporins, is essential for full expression of molecular function. J Biol Chem. 2011 Feb 4;286(5):3342-50.
69. Morishita Y, Hanawa S, Miki T, Sugase T, Sugaya Y, Chinda J, Iimura O, Tsunematsu S, Ishibashi K, Kusano E. The association of plasma prorenin level with an oxidative stress marker, 8-OHdG, in nondiabetic hemodialysis patients. Clin Exp Nephrol. 2011 Jan 15. 15(3):398-404
70. Morishita Y, Ohnishi A, Watanabe M, Ishibashi K, Kusano E. Establishment of acute kidney injury mouse model by 0.75% adenine ingestion. Ren Fail. 2011;33(10):1013-8.
71.Morishita Y, Watanabe M, Nakazawa E, Ishibashi K, Kusano E.The interaction of LFA-1 on mononuclear cells and ICAM-1 on tubular epithelial cells accelerates TGF-beta1-induced renal epithelial-mesenchymal transition. PLoS One. 2011;6(8):e23267.
72. Morishita Y, Yasui T, Numata A, Onishi A, Ishibashi K,Kusano E Aliskiren suppresses the renin-angiotensin-aldosterone system and reduces blood pressure and albuminuria in elderly chronic kidney disease patients with hypertension. Int J Nephrol Renovasc Dis. 5:125-132 (2012)
73.Morishita Y, Watanabe M, Hanawa S, Iimura O, Tsunematsu S, Ishibashi K, Kusano E Long-term effects of aliskiren on blood pressure and the renin-angiotensin-aldosterone system in hypertensive hemodialysis patients Int J Nephrol Renovasc Dis. 5:45-51. (2012)
74. Association of primary care physicians’ exercise habits and their age, specialty, and workplace J Multidiscip Healthc. 6: 409–414. (2013)
75. Medication-prescribing patterns of primary care physicians in chronic kidney disease. Clin Exp Nephrol.
76. Primary care physicians' own exercise habits influence exercise counseling for patients with chronic kidney disease: a cross-sectional study BMC Nephrology
77. Skeletal muscle loss was negatively associated with spKT/V and dialysis duration in hemodialysis patients Therapeutic Apheresis and Dialysis
78. Positive association of vigorous and moderate physical activity volumes with skeletal muscle mass but not bone density or metabolism markers in hemodialysis patients.International Urology and Nephrology